MiFert Receptivity

The Window of Implantation Challenge

Standard IVF protocols assume a universal window of implantation, typically scheduling embryo transfer on day 5 post-ovulation (P+5) for fresh cycles or after 5 days of progesterone (P+5) for frozen embryo transfer.

However, research reveals:

• 25-30% of women have a displaced window of implantation
• The receptive window may occur earlier (pre-receptive) or later (post-receptive) than standard timing
• Transferring an embryo outside the receptive window results in implantation failure, even with high-quality embryos

MiFert Receptivity identifies your patient's personalized window of implantation, enabling precisely timed embryo transfer.

How MiFert Receptivity Works

Technology: Transcriptomic Analysis

MiFert Receptivity analyzes the expression of 238 genes in the endometrial tissue, creating a molecular signature that definitively classifies the endometrium as:

• Pre-Receptive – Not yet ready; needs additional progesterone exposure
• Receptive – Optimal timing for embryo transfer
• Post-Receptive – Window has passed; transfer should have occurred earlier

This proprietary algorithm has been validated in multiple clinical studies with over 90% accuracy in determining endometrial receptivity status.

The MiFert Receptivity Process

Step 1: Mock Cycle Biopsy

• Endometrial biopsy performed during a hormone replacement therapy (HRT) "mock" cycle
• Timing: typically after 5 days of progesterone exposure (P+5)
• Mimics the conditions of a planned frozen embryo transfer
• Sample divided: one portion for Receptivity, one for Microbiome/Immunology if ordered

Step 2: Molecular Analysis

• 238-gene expression analysis performed
• Proprietary algorithm determines receptivity status
• Turnaround time: 13-15 days

Step 3: Personalized Transfer Protocol

• If Receptive (P+5): Standard transfer timing is optimal
• If Pre-Receptive: Embryo transfer delayed (e.g., P+6 or P+7)
• If Post-Receptive: Embryo transfer advanced (e.g., P+4)
• Patient's personalized window is identified with precision

Step 4: Optimized Embryo Transfer

• Subsequent frozen embryo transfer scheduled according to personalized timing
• Dramatically improved implantation rates in patients with displaced window

Sample Collection & Requirements

Biopsy Timing:

• Mock cycle (HRT protocol) mimicking planned transfer
• After 5 days of progesterone exposure (P+5) – standard testing day
• Can be adjusted if specific timing suspected

Sample Requirements:

• Endometrial tissue (not mucosa or blood)
• Divided into two portions:
• Blue tube (RNA preservative): Receptivity + Microbiome
• Red tube (protein preservative): Immunology

Patient Preparation:

• No sexual intercourse 48 hours prior
• Empty bladder before procedure
• Light meal recommended (prevent vasovagal response)

Biopsy Technique:

• Office procedure using Pipelle or similar cannula
• Extract endometrial tissue (not just mucosa)
• Ensure adequate sample volume

Storage & Shipping:

• Refrigerate at 4°C for minimum 4 hours post-collection
• Ship with cold condenser (pre-frozen 24 hours)
• Complete all patient identification on tubes